Reconstitution guide

What you'll need

• Bacteriostatic water (0.9% benzyl alcohol)
• Alcohol swabs
• A sterile syringe (an insulin syringe works well for smaller volumes)
• An approved sharps container for disposal

Step-by-step process

• Sanitize your workspace and hands before beginning.
• Wipe the rubber stopper on both the peptide vial and the bacteriostatic water vial with an alcohol swab and let it air-dry.
• Draw the intended volume of bacteriostatic water into a sterile syringe.
• Insert the needle through the peptide vial's stopper at a slight angle and inject the water slowly down the inside wall of the vial, rather than directly onto the powder, to minimize foaming.
• Gently swirl the vial until the powder is fully dissolved. Do not shake — agitation can damage the peptide structure.
• Label the vial with the reconstitution date and the resulting concentration.

Calculating concentration

Concentration (mg/mL) is the total peptide content of the vial divided by the volume of bacteriostatic water added. For example, a 5mg vial reconstituted with 2mL of bacteriostatic water yields a concentration of 2.5mg/mL. Reconstituting the same vial with more water produces a more dilute solution; less water produces a more concentrated one.

After reconstitution

Once reconstituted, peptides should be refrigerated and used within the timeframe noted on our storage & handling page. Avoid repeated freeze-thaw cycles and direct light exposure.

For research use only

This guide describes general laboratory reconstitution technique. Vera Laboratories products are sold strictly for in-vitro laboratory and research use by qualified individuals and institutions — not for human or animal consumption, and not intended to diagnose, treat, cure, or prevent any disease. See our research use policy for full terms.